By Derek Kinchington, Raymond F. Schinazi
Royal London institution of drugs, united kingdom. moment writer, Raymond F. Schinazi, is at Emory Univ., Decatur, GA. deals simple and medical researchers versions to guage compounds potent opposed to acute and protracted infections. contains finished assays, caliber trying out, and unpublished equipment. DNLM: Hepatitis, Viral, Human - drug remedy.
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Additional info for Antiviral Methods and Protocols
Store at –20°C. 0 µL of HBV standards (100 ng/mL) plus 5 µL of tracking dye and 14 µL of TE (per lane). HBV standards are made by performing separate BamHI and EcoRI digests of cloned HBV DNA. The digests are combined in equimolar amounts and stored at –20°C. This produces several HBV fragments (positive hybridization controls) as well as a non-HBV plasmid DNA fragment (negative hybridization control). 3 kb. This mixture serves as a positive and a negative hybridization control, a size standard, and a quantitation standard that occupies only one lane of a gel.
This mixture serves as a positive and a negative hybridization control, a size standard, and a quantitation standard that occupies only one lane of a gel. HBV media standards. 1. 15 cells and store frozen at –70°C. Pool aliquots as necessary (typically 1–2 L) and centrifuge at 7000g, 10 min to remove cellular debris. Add the supernatant to an Amicon “stirred cell” (8400 series) fitted with a YM100 membrane (cat. no. 13642) 400 mL at a time. 54 Schmidt and Korba 54. 55. 56. 57. 58. 59. 60. 61. 62.
48. 49. 50. 51. 52. 53. 53 Isopropanol. Ammonium acetate. 25% bromophenol blue, 30% glycerol in TE. TAE (Tris-acetate-EDTA running buffer). Agarose (standard low–mr). Plastic pipets (1 mL to 50 mL, sterile, individually wrapped). 5 in. Glass Pasteur pipets. 96-Well tissue culture plates. 24-Well tissue culture plates. Tissue culture flasks (75, 150 cm2). 10-, 250-, 500-µL pipet tips. 0 mL). 1-mL strip tubes in strips of 8 and 12 tubes (sterile) (Continental Laboratories, San Diego, CA). Dialysis membranes (12,000-mol-wt cutoff) for microdialysis apparatus (Gibco-BRL).
Antiviral Methods and Protocols by Derek Kinchington, Raymond F. Schinazi